The action of phytase releasing orthophosphate from phytic acid.How was an Enviropig created?The Enviropig was developed by the introduction of a transgene construct composed of the promoter segment of the murine parotid secretory protein gene and the Escherichia coli phytase gene () into a fertilized porcine embryo by pronuclear microinjection, and this embryo along with other embryos was surgically implanted into the reproductive tract of an estrous synchronized sow. After a 114 day gestation period, the sow farrowed and piglets born were checked for the presence of the transgene and for phytase enzyme activity in the saliva. When the mature genetically modified pig was crossed with a conventional pig, approximately half of the pigs contained the phytase transgene.
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